Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: The summary results inflammation cytokines level by ELISA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis reduced the expression of IgE in the serum of allergic asthma mice. ( A ) ELISA was used to measure the total IgE content (ng/ml) in the serum of mice. ( B ) ELISA was used to detect the content of OVA-specific IgE (ng/ml) in the serum of mice. All experiments were repeated in triplicate to average (n=10). ## p <0.001 vs. control; ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis regulated the balance of Th1/Th2-related cytokines in BALF and lung tissues. ( A–E ) ELISA was used to detect the contents of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in BALF. ( F–J ) QRT-PCR was used to detect the expression of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in lung tissues. β-actin served as a reference gene. All experiments were repeated in triplicate to average (n=10). # p <0.05, ## p <0.001 vs. control; * p <0.05, ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Control

Journal: Experimental dermatology

Article Title: Anti-interleukin-31-antibodies ameliorate scratching behaviour in NC/Nga mice: a model of atopic dermatitis.

doi: 10.1111/j.1600-0625.2008.00766.x

Figure Lengend Snippet: Figure 4. Serum concentrations of IL-13 (a) and IgE (b) in the different intervention groups, measured by ELISA at end-point. The treatment modalities were with anti-IL-31, albumin and no treatment. Data are presented given in box plots. No statistical differences between the groups were found for variables (a) and (b).

Article Snippet: Mouse IgE ELISA Quantitation kit (Bethyl Laboratories Inc., Montgomery, TX, USA, Catalog no. E90-115) and mouse-IL-13 Quantikine M-assay (R&D, Minneapolis, MN, USA, Catalog no. M1300C) were used as outlined by the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: The summary results inflammation cytokines level by ELISA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis reduced the expression of IgE in the serum of allergic asthma mice. ( A ) ELISA was used to measure the total IgE content (ng/ml) in the serum of mice. ( B ) ELISA was used to detect the content of OVA-specific IgE (ng/ml) in the serum of mice. All experiments were repeated in triplicate to average (n=10). ## p <0.001 vs. control; ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis regulated the balance of Th1/Th2-related cytokines in BALF and lung tissues. ( A–E ) ELISA was used to detect the contents of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in BALF. ( F–J ) QRT-PCR was used to detect the expression of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in lung tissues. β-actin served as a reference gene. All experiments were repeated in triplicate to average (n=10). # p <0.05, ## p <0.001 vs. control; * p <0.05, ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Control

Journal: Journal of Pharmacy & Pharmaceutical Sciences

Article Title: Effects of dual therapy with betamethasone and tetracycline in a NC/Nga mouse model of atopic dermatitis

doi: 10.3389/jpps.2023.11182

Figure Lengend Snippet: Effects of topically applied betamethasone and tetracycline on serum IgE concentration in NC/Nga mice with AD-like skin lesions. The serum total IgE levels on the 31st day of assessment were measured by ELISA. The results for each experimental group are expressed as means ± SEM ( n = 6). * p < 0.01 versus none, § p < 0.01 versus none, † p < 0.01 versus betamethasone, tetracycline.

Article Snippet: The total IgE concentration in the serum was measured by enzyme-linked immunosorbent assay (ELISA) using a mouse IgE ELISA kit (Cedarlane, ON, Canada).

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Pharmaceuticals (Basel, Switzerland)

Article Title: Callicarpa dichotoma Leaf Extract Alleviates Atopic Dermatitis through the Suppression of T Cells and Keratinocytes Activation.

doi: 10.3390/ph15101280

Figure Lengend Snippet: Figure 4. CDE reduces IgE production and mast cell infiltration in AD mice. (a) Levels of IgE and mite- specific IgE were analyzed by ELISA in serum isolated on day 28 after induction. (b) Representative photos of sections for each group through toluidine blue staining and evaluation of the number of mast cell infiltration into the ear tissues of each group. Results are expressed as mean ± SEM of five mice. * p < 0.05, versus the AD group.

Article Snippet: Mouse IgE ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Isolation, Staining

Journal: Indian Journal of Pharmacology

Article Title: Platycodon grandiflorus alleviates DNCB-induced atopy-like dermatitis in NC/Nga mice

doi: 10.4103/0253-7613.99306

Figure Lengend Snippet: Effect of PG extracts for Total IgE levels in serum and infiltrated the mast cells in the DNCB-induced AD-like skin lesion (A) IgE concentration of each mouse was measured after sacrifice by ELISA. Data are presented as mean ± SEM (n = 5). Normalcontrol (NC), postive control (PC), PG1 (DNCB + PG 300 mg/kg), PG2 (DNCB + PG 500 mg/kg), DEX (DNCB + dexamethasone 1.5 mg/kg). (B) The number of infiltrating mast cells in the tissue sections of the skin lesion was counted in five sites chosen randomly. PG (DNCB + PG 500 mg/ kg). * P < 0.05 vs . PC.

Article Snippet: The levels of total serum IgE were measured by a mouse IgE ELISA kit (Shibayagi, Japan), following the manufacturer's instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: Food & Nutrition Research

Article Title: A multistrain probiotic formulation attenuates skin symptoms of atopic dermatitis in a mouse model through the generation of CD4 + Foxp3 + T cells

doi: 10.3402/fnr.v60.32550

Figure Lengend Snippet: Serum IgE and inflammatory cytokine levels. Blood was obtained from NC/Nga mice at week 12 for which the IgE (a), IL-4 (b), IL-10 (c), IL-13 (d), IFN-γ (e), and IL-12p40 (f) serum levels were determined using ELISA. Results are expressed as the mean+SD ( n =12). ** p <0.01, *** p <0.001 versus the control group; ### p <0.001 versus the excipient group; §§§ p <0.001 versus the GI7-L group.

Article Snippet: IgE levels were determined using a mouse IgE ELISA kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Enzyme-linked Immunosorbent Assay